Department of Orthopaedic Surgery 3471 Fifth Avenue
Pittsburgh, PA 15213
Phone: 412-605-3203 Fax: 412-687-0802
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Research Faculty
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| Stem Cell Research Laboratory Faculty |
Orthopaedic Research Laboratory Faculty |
| NeuroMuscular Reasearch Laboratory Faculty |
Orthopaedic BioDynamics Laboratory Faculty |
| Orthopaedic Engineering and Sports Medicine Laboratory Faculty |
Sports Orthopaedic Research Laboratory Faculty |
| Clinical Research Faculty and Staff |
Molecular Therapy Laboratory |
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| Stem Cell Research Laboratory Faculty |
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Johnny Huard, PhD - Director
Henry J. Mankin Chair in Orthopaedic Surgery
Research Growth and Development Laboratory
Vice Chairman for Musculoskeletal Cellular Therapeutics
Johnny Huard, Ph.D., of the University of Pittsburgh is an Associate Professor in the Departments of Orthopaedic Surgery and Molecular Genetics and Biochemistry and Bioengineering, and also the Director of the Growth and Development Laboratory of Children’s Hospital of Pittsburgh. He was recently named the Henry J. Mankin, Endowed Chair in Orthopaedic Surgery Research. Dr. Huard is also the Deputy Director of the McGowan Institute for Regenerative Medicine (MIRM); Cellular Therapeutic Pillar and an Associate Director of the Pittsburgh Tissue Engineering Initiative (PTEI). Dr. Huard is co-founder of Cook MyoSite, Inc., a biotechnology company.
Dr. Huard’s main research focus continues to expand the possibilities of tissue engineering by unlocking the potential of gene therapy and adult stem cell research and transferring research findings into the development of effective treatments for damaged or diseased tissues as they relate to the musculoskeletal system. He has established international recognition for his research discoveries in this area. He has made significant preclinical advances in the isolation, identification and characterization of muscle-derived stem cells. He is currently using those findings to explore and develop cutting-edge treatment programs to address Duchenne muscular dystrophy (DMD) and a variety of orthopaedic diseases and injuries including muscle injuries and repair, and the regeneration of bone and articular cartilage. Dr. Huard’s research program is funded by a variety of sources including the National Institutes of Health, Muscular Dystrophy Association, as well as other private and public foundations. Last year Dr. Huard was recognized by University of Pittsburgh Chancellor Mark A. Nordenberg, as a recipient of the Chancellor’s Distinguished Research Award.
Burhan Gharaibeh, PhD - Research Assistant Professor
Dr. Gharaibeh’s main research main research interest is how to best track non-engineered (normal) adult stem cells in recipient tissues. One of the most common techniques used is fluorescent in situ hybridization (FISH). Dr. Gharaibeh has been using this method to track stem cells in gender mismatch and species mismatch experiments where the engraftment site is examined for donor cells by searching for Y chromosomal paint signal in female recipients or human cells in a mouse model. Dr. Gharaibeh is interested in cytogenetic phenomena like cellular fusion, heterokaryon formation, and endoreduplication. He is also interested in any impacts of the methods of isolation and culture of stem cells on their genetic instability by using classical and molecular cytogenetic techniques. Chromosomal numerical and structural abnormalities are recorded and metaphases are photographed and analyzed.
Dr. Gharaibeh is currently investigating muscle derived stem cells (MDSCs) from different mammalian species (including murine and porcine tissues) and the use of these cells in tissue engineering and regeneration projects in several collaborative projects.
Burhan Gharaibeh, PhD has accepted a secondary appointment of Bioengineering at the Swanson School of Engineering, Department of Bioengineering.
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| Orthopaedic Research Laboratory Faculty |
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James D. Kang, MD
UPMC Chair in Orthopaedic Spinal Surgery
Executive Vice Chairman for Clinical Services
Ferguson Laboratory for Orthopaedic and Spine Research
James D. Kang, MD is an orthopaedic spine surgeon with a career-long interest in the basic science and clinical treatment of intervertebral disc degeneration. (Intervertebral discs are specialized soft tissue structures—“cushions”—interposed between the vertebrae of the spinal column that help keep the spine flexible. Degeneration of intervertebral discs frequently leads to back pain as well as a host of pathologic and disabling conditions—e.g., “slipped disc”—that can necessitate invasive spinal fusion surgery.) As the Director of the Ferguson Laboratory for Orthopaedic Research, Dr. Kang is leading the development of novel “minimally invasive” therapies of intervertebral disc degeneration based on the latest gene transfer and stem cell technologies. Currently, Dr. Kang and his research team are performing comprehensive preclinical studies addressing feasibility, safety, and efficacy of these technologies. The results to-date have garnered much favorable international attention—strongly suggesting that cell and molecular approaches have excellent potential for making the translation from the laboratory to the clinic, and ultimately reducing the incidence of back pain and the need for spinal surgery. Dr. Kang is a past recipient of the highest societal awards for spinal research conferred by the leading international spine societies—the International Society for the Study of the Lumbar Spine (ISSLS), the Cervical Spine Research Society (CSRS), and the North American Spine Society (NASS).
Joon Y. Lee, MD
Ferguson Laboratory for Orthopaedic and Spine Research
Dr. Lee specializes in cervical, thoracic, and lumbar spine surgery in the greater Pittsburgh area. Dr. Lee graduated summa cum laude from University of California, Berkeley, and obtained his medical degree from Yale Medical School. He completed his residency in orthopaedic surgery at the University of Pittsburgh Medical Center, PA. He completed his fellowship in spine surgery at the Rothman Institute-Thomas Jefferson University in Philadelphia, PA. Dr. Lee performs spinal surgery using standard techniques and the latest cutting-edge surgical technologies. These include minimally invasive spine surgery, disc replacement, and microsurgical decompression. For certain spinal problems, these new techniques may allow faster recovery and eliminate the need for spinal fusion. Dr. Lee has been involved in research at the National Institutes of Health, Yale Medical School Research Laboratories, and the Orthopaedic Research Laboratory at the University of Pittsburgh. He has authored numerous articles and lectured extensively on topics such as cervical disc herniation, cervical myelopathy, cervical disc replacement, lumbar disc herniation, lumbar spinal stenosis, lumbar disc replacement, spinal fusion, spine trauma, microsurgical decompression, and non-fusion technologies. For more information you can go to: pittsburghspinesurgery.com.
Gwendolyn Sowa, MD, PhD
Dr. Gwendolyn Sowa received a PhD in Biochemistry in 1997 and a degree in medicine in 2000 from the University of Wisconsin in Madison, Wisconsin. She completed her physical medicine and rehabilitation residency at the Rehabilitation Institute of Chicago, Northwestern Medical School in 2004.
Gwendolyn Sowa, MD, PhD joined the University of Pittsburgh Departments of Physical Medicine and Rehabilitation and Orthopaedics in 2005 as an Assistant Professor.
She is Co-Director of the Ferguson Laboratory for Orthopaedic Research, and has an active research program investigating the role of mechanical forces in disc degeneration. Dr Sowa is an award winning researcher and has presented her findings at international conferences and symposia.
Nam Vo, PhD
Nam Vo received his PhD degree with Dr. Michael Chamberlin in Biochemistry from the University of California, Berkeley in 1998 and completed his Post-Doctoral training with Dr. Michael Lai at the University of Southern California in 2003. His Doctorate research involved detailed molecular analyses of the early stages of E. coli transcription. He continued his training by studying the mechanism and regulation of hepatitis C virus RNA replication, initially as a Postdoctoral Fellow and subsequently as an Assistant Specialist in Dr. Caroline Kane at the University of California at Berkeley. Dr. Vo also taught Introductory Microbiology at Contra Costa College in 2005-2006.
Dr. Vo joined the Department of Orthopaedic Surgery of UPMC as a Research Assistant Professor in 2006. He is currently working in the Ferguson Spine Research Laboratory to investigate the molecular and cellular processes responsible for intervertebral disc diseases, with special emphasis on understanding gene regulation of the major disc extracellular matrix constituents.
James H-C Wang, PhD
MechanoBiology Laboratory
Dr. James H-C. Wang is currently an Professor in the Departments of Orthopaedic Surgery, Bioengineering, and Mechanical Engineering at the University of Pittsburgh. He serves as the Director of the MechanoBiology Laboratory in the Department of Orthopaedic Surgery. Dr. Wang is also a Faculty Member at the McGowan Institute for Regenerative Medicine at the University of Pittsburgh.
Dr. Wang received his Bachelor of Science in Solid Mechanics in 1982 and his Master of Science in Experimental Biomechanics in 1989 from Tongji University. After working for nine years at the same university, he came to the United States in 1991 and studied orthopaedic biomechanics at the Department of Orthopaedic Surgery of the Texas Tech Health Science Center. Then, he went to the University of Cincinnati (UC), pursuing his Ph.D. in bioengineering. He spent the next four years at the UC School of Medicine’s Cell Biology Department, where he studied cell mechanobiology. After receiving his Ph.D., he worked as a post-doctoral fellow at Johns Hopkins Medical School and Washington University in St. Louis, where he studied the molecular signaling pathways that lead to actin cytoskeletal remodeling of human endothelial cells in response to dynamic mechanical stretching conditions. Following his research fellowships at these two institutions, Dr. Wang took a faculty position in the Department of Orthopaedic Surgery at the University of Pittsburgh School of Medicine in 1998 and has remained as a faculty member since then. One of Dr. Wang’s major research interests is elucidating the developmental mechanisms of tendinopathy using in vitro and in vivo model systems and enhancing the biological, biochemical, and biomechanical properties of healing tendons and ligaments using functional tissue engineering approaches. In addition, he is interested in the cellular and molecular mechanisms of how mechanical forces are transmitted to cells and translated into anabolic or catabolic cellular responses. In recent years, Dr. Wang has been working on developing new biotechnological tools such as micropost force sensor arrays (MFSA) and cell traction force microscopy (CTFM) for basic cell mechanobiology studies.
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| NeuroMuscular Reasearch Laboratory Faculty |
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Scott M. Lephart PhD, ATC - Director
Dr Scott Lephart is the director of the Neuromuscular Research Laboratory as well as Department Chair and Associate Professor of Sports Medicine and Nutrition and Assistant Professor within the Department of Orthopaedic Surgery at the University of Pittsburgh. He completed his undergraduate degree at Marietta College (Marietta, OH) and both his Master's and Doctoral degrees in Sports Medicine at the University of Virginia (Charlottesville, VA) in 1988. Dr. Lephart's research interests include musculoskeletal sports and female ACL injury prevention, neuromechanical contributions to upper extremity function, and injury prevention in golfers. |
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| Orthopaedic BioDynamics Laboratory Faculty |
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Scott Tashman, PhD
Associate Professor
Department of Orthopaedic Surgery
Orthopaedic Biodynamics Laboratory
Orthopaedic Biodynamics Laboratory
Rivertech Office Works
3820 South Water Street
Pittsburgh, PA 15203
Telephone: 412.586.3950
Fax: 412.586.3979
Email: tashman@pitt.edu |
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| Contact Information | Lori Freund Research Administrator 412.586.3960 freundl@upmc.edu Rivertech Office Works 3820 South Water St. Pittsburgh, PA 15203 | | |
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| Orthopaedic Engineering and Sports Medicine Laboratory Faculty |
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Patrick Smolinski, PhD
Associate Professor, Department of Mechanical Engineering
Associate Faculty, Department of Bioengineering
Associate Faculty, Department of Orthopaedic Surgery
University of Pittsburgh
Department of Biomechanical Engineering
Benedum Hall – 631
3700 O’Hara Street
Pittsburgh, PA 15261
Telephone: 412-624-9788
Fax: 412-624-4846
Email: patsmol@pitt.edu
Research Interests
Dr. Smolinski’s primary research interests include the development of new computational simulation methods and the application of computational methods to problems in manufacturing and biomechanics. He is currently involved with research projects in the extended finite method for modeling solidification processes, the finite element modeling of sheet metal forming, modeling the flow of granular material, stress analysis and design of ankle replacement components, and the study of the wear of polyethylene joint components.
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| Sports Orthopaedic Research Laboratory Faculty |
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Christopher D. Harner, MD
Blue Cross Professor of Western Pennsylvania
Medical Director, UPMC Center for Sports Medicine
Department of Orthopaedic Surgery
UPMC Center for Sports Medicine
3200 South Water Street
Pittsburgh, PA 15203
Telephone: 412-432-3600
Fax: 412-432-3690
Email: harnercd@upmc.edu
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| Clinical Research Faculty and Staff |
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Dr. James J. Irrgang; PhD PT ATC
Director of Clinical Research
Dr. Irrgang’s personal research interests include:
• Development and validation of patient-oriented outcome measures
• Evidence-based practice
• Clinical research related to diagnosis, treatment and prognosis of musculoskeletal conditions involving the knee and shoulder
The clinical research staff includes Research Coordinators and Clinical Research Assistants. Ms. Kimberly Francis serves as the Research Coordinator for the Division of Sports Medicine and Ms. Alma Heyl serves as the Research Coordinator for the Divisions of Adult Reconstruction and Musculoskeletal Oncology. Within their respective Divisions, the Research Coordinators are responsible for establishing and maintaining a patient registry, obtaining and maintaining IRB approval for clinical research studies, subject recruitment, obtaining informed consent, assisting with data collection, data entry, managing administrative and compliance issues and providing supervision for the Research Assistants.
The Research Assistants are responsible for enrolling new patients into the research registry, instructing patients in the use of the computerized patient-intake system, collecting and recording examination data, recording surgical findings and procedures, entering clinical and surgical data into the computerized clinical data repository, maintaining a schedule to collect follow-up data, identifying and screening subjects for participation in ongoing clinical research studies and ensuring that all clinical research activities are performed in compliance with HIPAA and IRB regulations. The current Research Assistants within the Department of Orthopaedic Surgery are Nnamdi Okeke and Sarah Henry, who are third year medical students that are completing a year-long Clinical Research Fellowship and Gregory Bonci.
Dr. Irrgang’s personal research interests include:
• Development and validation of patient-oriented outcome measures
• Evidence-based practice
• Clinical research related to diagnosis, treatment and prognosis of musculoskeletal conditions involving the knee and shoulder
The clinical research staff includes Research Coordinators and Clinical Research Assistants. Ms. Kimberly Francis serves as the Research Coordinator for the Division of Sports Medicine and Ms. Alma Heyl serves as the Research Coordinator for the Divisions of Adult Reconstruction and Musculoskeletal Oncology. Within their respective Divisions, the Research Coordinators are responsible for establishing and maintaining a patient registry, obtaining and maintaining IRB approval for clinical research studies, subject recruitment, obtaining informed consent, assisting with data collection, data entry, managing administrative and compliance issues and providing supervision for the Research Assistants.
The Research Assistants are responsible for enrolling new patients into the research registry, instructing patients in the use of the computerized patient-intake system, collecting and recording examination data, recording surgical findings and procedures, entering clinical and surgical data into the computerized clinical data repository, maintaining a schedule to collect follow-up data, identifying and screening subjects for participation in ongoing clinical research studies and ensuring that all clinical research activities are performed in compliance with HIPAA and IRB regulations. The current Research Assistants within the Department of Orthopaedic Surgery are Nnamdi Okeke and Sarah Henry, who are third year medical students that are completing a year-long Clinical Research Fellowship and Gregory Bonci.
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| Contact Information | James J. Irrgang; PhD PT ATC Director of Clinical Research 412.605.3351 irrgangjj@upmc.edu Suite 911 Kaufmann Medical Building,
3471 Fifth Avenue,
Pittsburgh, Pa 15213 | | |
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| Molecular Therapy Laboratory |
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The established molecular therapy laboratory, under the direction of Bing Wang MD., PhD., has interest focused on the virology of adeno-associated virus (AAV) and its utility as a novel gene therapy vector. In contrast to other viral vectors, recombinant adeno-associate virus (rAAV) is a promising gene replacement vector. It has demonstrated the best gene transfer efficiency and longevity among all viral and non-viral vectors tested in muscle tissue.
In past years, Dr. Wang has been engaging in the projects of gene therapy for neuromuscular disorders, specifically Duchenne and Limb Girdle muscular dystrophies, using AAV viral vectors as gene vehicles. Deficiency of dystrophin protein causes Duchene muscular dystrophy (DMD), a most common disabling and lethal disease without treatment currently available. We first showed that AAV vectors carrying the human mini-dystrophin genes achieved efficient and stable correction of major biochemical and physiological defects of dystrophic muscle [1]. The further studies showed that AAV-mini-dystrophin gene treatment also improved mdx muscle contractile function [2]. Currently, the systemic human mini-dystrophin gene transfer by AAV vector intraperitoneal injection (i.p.) has been found to improve functions and life-span of dystrophin and utrophin double knockout (DKO) mice [3]. We discovered that the long-term expression of human mini-dystrophin and therapeutic benefits in transgenic mdx mice [4]. Importantly, the prolonged life-span was also observed in transgenic dystrophin/utrophin DKO mice. The large animal model is clinically and biologically superior to mdx model. We have first tested the biological functions of the AAV-mediated canine mini-dystrophin in the mdx mouse model [5]. The results indicated that the highly efficient canine mini-dystrophin was expressed in muscle tissue. Also, it could improve pathology and protect myofiber membrane integrity of dystrophic muscles. In a word, these experiments demonstrate that AAV mediated the human or canine mini-dystrophin ameliorates dystrophic muscle functions and life-span in rodent model. It provides the evidence of feasibility for DMD gene therapy.
Beside Duchene muscular dystrophy, the research plan in long term of molecular therapy laboratory is involving in the gene therapy for the joint/tendon injury, muscle/nerve injures, skeletal muscle repair and age related bone lose/muscle atrophy. Currently, we cooperated with the other laboratories and made AAV constructs, such as AAV-sFLT1, AAV-BMP4, AAV-Decorin, AAV-MPRO, AAV-MMP1, AAV-IKBSR, AAV- cFLIP (University of Pittsburgh) and AAV-Osteoprotegerin (Wayne State University). Also, the lab is actively engaged in multi-applications of AAV vectors for gene therapy: 1)Therapeutic gene packaging in self-complimentary AAV vectors, 2) siRNA in AAV vectors, 3) Tet on/Tet off AAV vector, 4) Differrent serotype AAV vector purification and application.
Base on the equipped facilities for AAV vector gene transfer studies and molecular biology, the molecular therapy laboratory is also a Vector Core for the projects involved in viral vectors development for gene therapy. The goal of this core is to construct vectors such as adeno-associated, adenoviral- and retoviral vectors for the projects.
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